A Microflow Cytometry-Based Agglutination Immunoassay for Point-of-Care Quantitative Detection of SARS-CoV-2 IgM and IgG

Section 1: Publication

Publication Type

Authorship

Jianxi Qu, Mathieu Chenier, Yushan Zhang and Chang-qing Xu

Title

A Microflow Cytometry-Based Agglutination Immunoassay for Point-of-Care Quantitative Detection of SARS-CoV-2 IgM and IgG

Year

2021

Publication Outlet

Micromachines 2021, 12, 433

DOI

https://doi.org/10.3390/mi12040433

ISBN

ISSN

Citation

Jianxi Qu, Mathieu Chenier, Yushan Zhang and Chang-qing Xu, A Microflow Cytometry-Based Agglutination Immunoassay for Point-of-Care Quantitative Detection of SARS-CoV-2 IgM and IgG, Micromachines 2021, 12, 433. https://doi.org/10.3390/mi12040433.

Abstract

A rapid, sensitive and simple microflow cytometry-based agglutination immunoassay (MCIA) was developed for point-of-care (POC) quantitative detection of SARS-CoV-2 IgM and IgG antibodies. The antibody concentration was determined by using the transit time of beads aggregates. A linear relationship was established between the average transit time and the concentration of SARS-CoV-2 IgM and IgG, respectively. The limit of detection (LOD) of SARS-CoV-2 IgM and IgG by the MCIA measurement are 0.06 mg/L and 0.10 mg/L, respectively. The 10 µL sample consumption, 30 min assay time and the compact setup make this technique suitable for POC quantitative detection of SARS-CoV-2 antibodies.

Plain Language Summary

Section 2: Additional Information

Program Affiliations

GWF: Global Water Futures

Project Affiliations

GWF-SSSWQM: Sensors and Sensing Systems for Water Quality Monitoring

Submitters

Name	Role	Email	Institution

Publication Stage

Theme

Presentation Format

Additional Information

Sensor and Sensing Systems for Water Quality Monitoring 2, Refereed Publications