Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment

Section 1: Publication

Publication Type

Authorship

Lingzi Ma, Zhicheng Huang and Juewen Liu

Title

Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment

Year

2021

Publication Outlet

Chemical Communications, 57, 7641-7644

DOI

https://doi.org/10.1039/D1CC03102C

ISBN

ISSN

Citation

Lingzi Ma, Zhicheng Huang and Juewen Liu, Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment. Chemical Communications, 57, 7641-7644, https://doi.org/10.1039/D1CC03102C, 2021.

Abstract

A new self-cleaving ribozyme was obtained from in vitro selection, displaying site-specific cleavage activity under denaturing conditions, such as high temperatures up to 95 °C, and denaturing solvents (20 M formamide). Adding salt such as Mg2+ also inhibited its activity. The conserved ribozyme sequence was found in the genome of several extremophiles, suggesting its potential biological relevance. This study provides an example of a ribozyme working under exotic conditions, which may expand the application of ribozymes in non-biological environments.

Plain Language Summary

Section 2: Additional Information

Program Affiliations

GWF: Global Water Futures

Project Affiliations

GWF-SSSWQM: Sensors and Sensing Systems for Water Quality Monitoring

Submitters

Name	Role	Email	Institution

Publication Stage

Theme

Presentation Format

Additional Information

Sensor and Sensing Systems for Water Quality Monitoring 2, Refereed Publications