Transit time and amplitude thresholds in a microflow cytometer for particle classification

https://doi.org/10.1109/pn50013.2020.9167024

Zhang, Y., Xu, C. Q. (2020) The impact of transit time on a microflow cytometer for particle classification. 2020 Photonics North (PN), pp. 1-1, https://doi.org/10.1109/PN50013.2020.9167024 .

Microflow cytometers and many other miniaturized microfluidic devices have shown great potential in many fields, such as, particle detection, cell sorting and classification. A reliable signal analysis method is required to improve the measurement accuracy of the emerging microfluidic devices. In this paper, a novel method is presented to analyze the signal from microspheres with different diameters based on transit time and amplitude. Experimental results show that transit time threshold plays a more important role at lower flow rate for particle differentiation and can be used to improve the performance of a microflow cytometer.