Thermal Drying Synthesized Density-Tunable and High-Stability Nonthiolated Spherical Nucleic Acids for Split Aptamer and Lateral Flow Biosensors

Section 1: Overview

Dataset Title

Thermal Drying Synthesized Density-Tunable and High-Stability Nonthiolated Spherical Nucleic Acids for Split Aptamer and Lateral Flow Biosensors

Dataset DOI

https://www.frdr-dfdr.ca/repo/dataset/8f276cdb-f622-4d34-8cba-3a260758959e

Related Paper DOIs (one per line)

https://doi.org/10.1016/j.bios.2025.117863

Abstract, Purpose, and/or Plain Language Summary

This dataset provides fluorescence assay data demonstrating the tunable DNA density of nonthiolated spherical nucleic acids (SNAs). These nonthiolated SNAs were then employed for oxytetracycline detection using UV-vis spectroscopy and for detecting genetically modified soybean using lateral flow assays (LFAs). Signal acquisition was performed with a Tecan Spark multifunctional microplate reader (https://www.tecan.com/spark-overview), with excitation/emission wavelengths set at 467/525 nm for SYBR Green I and 485/520 nm for carboxyfluorescein. Quantitative analysis of the LFA signal intensity data was performed using ImageJ software (https://fiji.sc/).

Program Affiliations

GWFO: Global Water Futures Observatories

Creators and Contributors

Name	Role	Email	Institution

Keywords

Biosensors Aptamer Spherical Nucleic Acids

Additional Information

CFI-MSI Project No. 42687
Major Science Initiative
Global Water Futures Observatories

Section 2: Access and Downloads

Access to the Dataset

Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

Does the data have access restrictions?

▣ No restriction (data is currently open to public)

◻ Limited (data is currently under embargo until publication)

◻ Limited (data involves intellectual property issues related to local or traditional knowledge)

◻ Limited (release of data may cause harm to the environment or to the public)

◻ Limited (pre-existing data has been used and is subject to access restrictions)

◻ Limited (data involves human subjects)

◻ Limited (data is supported by industry partnerships)

◻ Limited (data is supported by government partnerships)

Datasets and Real-time Data Feeds

Download Links and Instructions

https://www.frdr-dfdr.ca/repo/dataset/8f276cdb-f622-4d34-8cba-3a260758959e

Total Size of all Dataset Files (GB)