Editing Potential Use of Environmental DNA to Monitor Brook Trout Population Dynamics, Washington Creek, Ontario

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Stream water collections were conducted on monthly basis to determine seasonal fluctuations in brook trout eDNA. This was contrasted with traditional surveying methods (i.e., electrofishing) with the final goal to explore whether eDNA has a comparable potential to detect seasonal changes in fish biomass and abundance.

Washington Creek stream data – collected using a GPS device to determine exact site coordinates, stream width and depth were measured with a measuring tape and a meter stick, respectively, velocity was determined with a velocity meter; 
Washington Creek fish numbers and species identification – Smith-Root electrofishing backpack unit was used for fish collection that were immediately identified by experienced surveyors;  
Fish fork length, fish whole body weight – determined using a fish measuring board (cm), whereas body weight was measured using a scale (g); 
Washington Creek brook trout quantitative polymerase data (cycle threshold – Ct values), and Washington Creek brook trout eDNA copy numbers – a TaqMan based assay with species specific primers targeting brook trout mitochondrial cytochrome b were used for qPCR amplification.

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